Article

Development of a multiplex and semi-nested PCR assay for detection of Yersinia enterocolitica and Aeromonas hydrophila in raw milk

2000-04-01

Abstract excerpt

Single-locus (sl), multiplex (m), and semi-nested (sn) polymerase chain reaction (PCR) procedures were developed for the detection of Yersinia enterocolitica and Aeromonas hydrophila in raw milk samples. Two oligonucleotide primers for each pathogen were used in PCR, to detect the yst gene of Y. enterocolitica and aer gene of A. hydrophila. The amplified fragment size was 145 base-pair (bp) for yst gene, and 209 b...

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Identifiers and source

Literature Corpus work: b78916b4-5291-5168-9534-687ea319c8de
DOI: 10.1006/fmic.1999.0298

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