Article

A framework for RNA quality correction in differential expression analysis

2016-09-09

Abstract excerpt

RNA sequencing (RNA-seq) is a powerful approach for measuring gene expression levels in cells and tissues, but it relies on high-quality RNA. We demonstrate here that statistical adjustment employing existing quality measures largely fails to remove the effects of RNA degradation when RNA quality associates with the outcome of interest. Using RNA-seq data from a molecular degradation experiment of human brain tiss...

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Literature Corpus work: b3824686-a160-5509-b19f-c71ff040597c
DOI: 10.1101/074245

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