Article
Discovery of pathogenicity genes in the crucifer anthracnose fungus Colletotrichum higginsianum, using random insertional mutagenesis.
Molecular plant-microbe interactions : MPMI - 1 Feb 2009
Huser Aurélie, Takahara Hiroyuki, Schmalenbach Wolfgang, O'Connell Richard
Abstract excerpt
Agrobacterium tumefaciens-mediated transformation (ATMT) was used for random insertional mutagenesis to identify pathogenicity genes in the hemibiotrophic fungus Colletotrichum higginsianum. A high-throughput primary infection assay on Arabidopsis thaliana seedlings allowed the rapid screening of 8,850 transformants. Forty mutants showing reproducible pathogenicity defects on Arabidopsis and Brassica plants were...
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