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Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus

31 Jan 2020

Abstract excerpt

Abstract Background Quantitative PCR (qPCR) is a powerful tool that is particularly well-suited to measure mRNA levels in clinical samples, especially those with relatively low cell counts. However, a caveat of this approach is that reliable, stably expressed reference (housekeeping) genes are vital in order to ensure reproducibility and appropriate biological inference. In this study, we evaluated the expression...

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PMID: 32005148
DOI: 10.1186/s12865-020-0334-8

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