Article

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

2016-03-22

Abstract excerpt

Retroviruses exhibit signature integration preferences on both the local and global scales. Here, we present a detailed protocol for (1) generation of diverse libraries of retroviral integration sites using ligation-mediated PCR (LM-PCR) amplification and next-generation sequencing (NGS), (2) mapping the genomic location of each virus-host junction using BEDTools, and (3) analyzing the data for statistical relevan...

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Literature Corpus work: a318d8df-aa58-58ae-86ad-bde93aba9d65
DOI: 10.3791/53840-v

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