Article

Rapid and homogenous detection of Apple stem pitting virus by RT-PCR and a fluorogenic 3' minor groove binder-DNA probe

2002-10-01

Abstract excerpt

Real-time PCR with fluorogenic hydrolysis probes (5' nuclease assay) is increasingly used for the detection of pathogens for diagnostic purposes. Nevertheless, the size of the probes, usually 25-40 nucleotides, might limit their use to detect pathogens with high genome variability between isolates, where an identical sequence cannot be found without multiple mismatches. In this report, we describe a 5' nuclease as...

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Literature Corpus work: a034c860-a5f9-5ca6-88bb-8b3db3a1eb22
DOI: 10.1023/a:1020849304902

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