Article
Rapid differentiation of citrus Hop stunt viroid variants by real-time RT-PCR and high resolution melting analysis.
Molecular and cellular probes - 1 Jan 2000
Loconsole Giuliana, Onelge Nuket, Yokomi Raymond K, Kubaa Raied Abou, Savino Vito, Saponari Maria
Abstract excerpt
The RNA genome of pathogenic and non-pathogenic variants of citrus Hop stunt viroid (HSVd) differ by five to six nucleotides located within the variable (V) domain referred to as the "cachexia expression motif". Sensitive hosts such as mandarin and its hybrids are seriously affected by cachexia disease. Current methods to differentiate HSVd variants rely on lengthy greenhouse biological indexing on Parson's...
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