Article

Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease

2009-04-01

Abstract excerpt

In this study a spot nested RT-PCR assay was developed for the detection of Apple stem pitting virus (ASPV). A one step RT-PCR for the generic detection of foveaviruses using degenerate primers that target a conserved region of the RNA-dependent RNA polymerase (RdRp) gene was followed by a nested PCR that amplifies a 312 bp ASPV specific product. The method is rapid, simple and displays high sensitivity and broad...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c0475ae0-1001-5327-a317-c4201338ce90
DOI: 10.1111/j.1365-3059.2008.01952.x

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation diseaseDOI 10.1111/j.1365-3059.2008.01952.xRead

Cydonia japonica, Pyrus calleryana and P. amygdaliformis: three new ornamental or wild hosts of Apple stem pitting virus.Semantic proximity Read

Occurrence and genetic diversity analysis of apple stem pitting virus isolated from apples in China.Semantic proximity Read

First Report on Detection of Three Bunya-Like Viruses in Apples in BrazilSemantic proximity Read

First Report of Apple Luteovirus 1 in Apple Trees in GreeceSemantic proximity Read

First Report of Peach-associated luteovirus in Nectarine (Prunus persica) in ItalySemantic proximity Read

Fast and sensitive on-site isothermal assay (LAMP) for diagnosis and detection of three fruit tree phytoplasmasSemantic proximity Read

Rapid and homogenous detection of Apple stem pitting virus by RT-PCR and a fluorogenic 3' minor groove binder-DNA probeSemantic proximity Read

Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in TaiwanSemantic proximity Read

Detection and molecular characterization of Italian Grapevine rupestris stem pitting-associated virus isolatesSemantic proximity Read

Passion Fruit Green Spot Virus Genome Harbors a New Orphan ORF and Highlights the Flexibility of the 5′-End of the RNA2 Segment Across CilevirusesSemantic proximity Read

First Report of Apple Stem Grooving Virus in Yunnan Huang Jing (Polygonatum kingianum) in ChinaSemantic proximity Read

Molecular Characterization and Detection of African Oil Palm Ringspot VirusSemantic proximity Read