Article

Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific Manner

2022-08-29

Abstract excerpt

Standard cell culture is routinely performed at supraphysiological oxygen concentrations (~18% O2). Conversely, oxygen levels in most tissues range from 1%–6% (physioxia). Such hyperoxic conditions can alter reactive oxygen species (ROS) production, energy metabolism, mitochondrial network dynamics, and response to drugs and hormones. The aim of this project was to investigate the transcriptional response to...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9f9cc34a-0a17-5a74-a4b1-2a5ba4e56872
DOI: 10.20944/preprints202208.0497.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific MannerDOI 10.20944/preprints202208.0497.v1Read

Culture of Cancer Cells at Physiological Oxygen Levels Affects Gene Expression in a Cell-Type Specific MannerSemantic proximity Read

Physiologically Relevant Oxygen Concentration (6% O2) as an Important Component of the Microenvironment Impacting Melanoma Phenotype and Melanoma Response to Targeted Therapeutics In VitroSemantic proximity Read

Oxygen in the cultivation of stem cells.Semantic proximity Read

Altered cellular redox homeostasis and redox responses under standard oxygen cell culture conditions versus physioxiaSemantic proximity Read

Physioxia Reprograms Glioblastoma Cells Enhancing Migration and Altering Therapeutic SensitivitySemantic proximity Read

Platform for combined analysis of functional and biomolecular phenotypes of the same cell.Semantic proximity Read

Basic Biology of Hypoxic Responses Mediated by the Transcription Factor HIFs and Its Implication for MedicineSemantic proximity Read

Reactive oxygen species (ROS) are a key determinant of cancer's metabolic phenotype.Semantic proximity Read

Derivation of Breast Cancer Cell Lines Under Physiological (5%) Oxygen ConcentrationsSemantic proximity Read

Longitudinal dynamics of the tumor hypoxia response: From enzyme activity to biological phenotype.Semantic proximity Read

Cell Metabolism Under Microenvironmental Low Oxygen Tension Levels in Stemness, Proliferation and PluripotencySemantic proximity Read

Transcriptome Alterations Due to Physiological Normoxic (2% O 2 ) Culture of Human Embryonic Stem CellsSemantic proximity Read