Article

Improved Genome Packaging Efficiency of AAV Vectors Using Rep Hybrids

2021-05-11

Abstract excerpt

Recombinant Adeno-associated viruses (rAAVs) are one of the most commonly used vectors for a variety of gene therapy applications. In the last two decades research focused primarily on the characterization and isolation of new cap genes resulting in hundreds of natural and engineered AAV capsid variants while the rep gene, the other major AAV open reading frame, has been less studied. This is due to the fact tha...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9d29b320-ec4d-504b-b30b-270aba77873d
DOI: 10.1101/2021.05.10.443533

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Improved Genome Packaging Efficiency of AAV Vectors Using Rep HybridsDOI 10.1101/2021.05.10.443533Read

AAV- based vector improvements unrelated to capsid protein modificationSemantic proximity Read

Evidence for Packaging of rep-cap Sequences into Adeno-Associated Virus (AAV) Type 2 Capsids in the Absence of Inverted Terminal Repeats: a Model for Generation of rep -Positive AAV ParticlesSemantic proximity Read

High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and CapSemantic proximity Read

Self-Complementary Recombinant Adeno-Associated Viral Vectors: Packaging Capacity And The Role of Rep Proteins in Vector PuritySemantic proximity Read

Identification and elimination of replication-competent adeno-associated virus (AAV) that can arise by nonhomologous recombination during AAV vector productionSemantic proximity Read

Analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene productsSemantic proximity Read

Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and CapSemantic proximity Read

Comprehensive mutagenesis maps the effect of all single-codon mutations in the AAV2 rep gene on AAV production.Semantic proximity Read

Novel Tools for Production and Purification of Recombinant Adenoassociated Virus VectorsSemantic proximity Read

Adeno-associated virus (AAV2) can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virusSemantic proximity Read

Detailed Protocol for the Novel and Scalable Viral Vector Upstream Process for AAV Gene Therapy ManufacturingSemantic proximity Read

Deciphering Key Adenoviral Elements in the Production of Recombinant Adeno-Associated Virus VectorsSemantic proximity Read