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Article

Novel Tools for Production and Purification of Recombinant Adenoassociated Virus Vectors

10 Dec 1998

Abstract excerpt

Standard protocols for the generation of adenoassociated virus type 2 (AAV-2)-based vectors for human gene therapy applications require cotransfection of cells with a recombinant AAV (rAAV) vector plasmid and a packaging plasmid that provides the AAV rep and cap genes. The transfected cells must also be overinfected with a helper virus, e.g., adenovirus (Ad), which delivers multiple helper functions necessary for...

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Identifiers

PMID: 9874273
DOI: 10.1089/hum.1998.9.18-2745

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