Article

Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein

2014-06-01

Abstract excerpt

Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈10⁸/ml) were obtained from mycelial cultures from five isolat...

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Literature Corpus work: 125b5398-72d2-5877-96d0-83f89cec4ff1
DOI: 10.1007/s10658-013-0301-9

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