Article

Recognition of cellular RNAs by the S9.6 antibody creates pervasive artefacts when imaging RNA:DNA hybrids

2020-01-13

Abstract excerpt

<h4>ABSTRACT</h4> The contribution of RNA:DNA hybrid metabolism to cellular processes and disease states has become a prominent topic of study. The S9.6 antibody recognizes RNA:DNA hybrids with a subnanomolar affinity, making it a broadly used tool to detect and study RNA:DNA hybrids. However, S9.6 also binds double-stranded RNA in vitro with significant affinity. Though frequently used in immunofluorescence mic...

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Literature Corpus work: 907bad1c-0c66-51e1-8d37-9b4ee49e6096
DOI: 10.1101/2020.01.11.902981

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