Article

Combining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification

2024-05-03

Abstract excerpt

Data Independent Acquisition (DIA) is increasingly preferred over Data Dependent Acquisition (DDA) due to its higher throughput and fewer missing values. Whereas DDA often utilizes stable isotope labeling to improve quantification, DIA mostly relies on label-free approaches. Efforts to integrate DIA with isotope labeling include chemical methods like mTRAQ and dimethyl labeling, which, while effective, complicate...

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Literature Corpus work: 8f4dceee-1515-5556-99e7-bde03419e8f6
DOI: 10.1101/2024.05.03.592381

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