Article
Quantitative Proteomic Analysis in Candida albicans Using SILAC-Based Mass Spectrometry.
Proteomics - 1 Mar 2018
Kaneva Iliyana N, Longworth Joseph, Sudbery Peter E, Dickman Mark J
Abstract excerpt
Stable isotope labelling by amino acids in cell culture (SILAC) in conjunction with MS analysis is a sensitive and reliable technique for quantifying relative differences in protein abundance and posttranslational modifications between cell populations. We develop and utilise SILAC-MS workflows for quantitative proteomics in the fungal pathogen Candida albicans. Arginine metabolism provides important cues for...
Topics
- Candida albicans
- Fungal Proteins
- Isotope Labeling
- Mass Spectrometry
- Mutation
- Phosphoprotein Phosphatases
- Proteome
