Article

High-resolution ion mobility as an alternative to quadrupole-based precursor isolation for reducing chimeric fragmentation spectra in bottom-up proteomics

2025-05-22

Abstract excerpt

To assess the potential for high resolution ion mobility (HRIM) as an alternative means of precursor isolation for mass spectrometry fragmentation analysis we performed a meta-analysis of predicted tryptic peptide features from the human proteome to measure the rate of chimeric spectrum generation relative to traditional quadrupole-based isolation. Results indicate that for proteomic mixtures, HRIM separation with...

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Literature Corpus work: 8dbe6b07-ddce-533f-8ebc-65967bc0d1d7
DOI: 10.22541/au.174793709.95097372/v1

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