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Flow Cytometric Analysis and Sorting of Enteric Nervous System Cells: From Human to Mouse, an Optimized Protocol

2025-03-11

Abstract excerpt

Isolation of neurons and glial cells from the enteric nervous system (ENS) enables ex-vivo studies, including analysis of genomic and transcriptomic profiles. While we previously reported a fluorescence activated cell sorting (FACS)-based isolation protocol for human ENS cells, no equivalent exists for mice. As directly applying the human protocol to mouse tissue, resulted in low recovery of live ENS cells, we com...

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Literature Corpus work
8c54f32d-430d-5d0f-bc72-771d9d79a407
DOI
10.20944/preprints202503.0749.v1
Open publication

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Flow Cytometric Analysis and Sorting of Enteric Nervous System Cells: From Human to Mouse, an Optimized ProtocolDOI 10.20944/preprints202503.0749.v1
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