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Flow Cytometric Analysis and Sorting of Murine Enteric Nervous System Cells: An Optimized Protocol

18 May 2025

Abstract excerpt

Isolation of neurons and glia from the enteric nervous system (ENS) enables ex vivo studies, including the analysis of genomic and transcriptomic profiles. While we previously reported a fluorescence-activated cell sorting (FACS)-based isolation protocol for human ENS cells, no equivalent exists for mice. As directly applying the human protocol to mouse tissue resulted in low recovery of live ENS cells, we...

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PMID: 40429965
DOI: 10.3390/ijms26104824

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