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Stable and assayable polymicrobial human airway model reveals complex interactions between <i>Pseudomonas aeruginosa</i> and lung commensals

2025-10-30

Abstract excerpt

Polymicrobial-host crosstalk shapes airway barrier integrity and inflammation, yet standard in vitro systems rarely sustain interaction-dependent phenotypes. We engineered an aqueous two-phase system (ATPS) confined bronchial co-culture that stabilized day-scale assays while maintaining epithelial function. A 16HBE14o-/HUVEC cell insert model was challenged with Pseudomonas aeruginosa PA01, Streptococcus pneumo...

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Literature Corpus work
7ed58581-1f7c-55f5-be36-6471a627ed7f
DOI
10.1101/2025.10.30.685207
Open publication

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Stable and assayable polymicrobial human airway model reveals complex interactions between <i>Pseudomonas aeruginosa</i> and lung commensalsDOI 10.1101/2025.10.30.685207
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