Back to search

Article

Sanger sequencing-the gatekeeper to exclude false positives in nucleic acid-based diagnostics for infectious diseases

2025-12-12

Abstract excerpt

<title>Abstract</title> <p> Background False-positive results are a known challenge in polymerase chain reaction (PCR)-based diagnostics for infectious diseases. The widespread public testing during the COVID-19 pandemic brought the issue to unprecedented global attention with immense clinical and societal consequences. Most authors of scientific publications claim contamination due to poor laboratory managemen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
783e5958-5c1b-569b-a174-afb9401408bb
DOI
10.21203/rs.3.rs-8271122/v1
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Sanger sequencing-the gatekeeper to exclude false positives in nucleic acid-based diagnostics for infectious diseasesDOI 10.21203/rs.3.rs-8271122/v1
Overhauling a faulty control in the CDC-recommended SARS-CoV-2 RT-PCR test panelSemantic proximity
Development of a sensitive molecular diagnostic assay for detecting <i>Borrelia burgdorferi</i> DNA from blood of Lyme disease patients by digital PCRSemantic proximity
Advances in Genotyping Detection of Fragmented Nucleic Acids.Semantic proximity
Patient DNA cross-reactivity of the CDC SARS-CoV-2 extraction control leads to an inherent potential for false negative resultsSemantic proximity
Development of a sensitive molecular diagnostic assay for detecting Borrelia burgdorferi DNA from the blood of Lyme disease patients by digital PCRSemantic proximity
<i>In silico</i> analyses identifies sequence contamination thresholds for Nanopore-generated SARS-CoV2 sequencesSemantic proximity
Protocol for DNA Extraction from QuantiFERON-TB Gold Tubes for PCR and Sequencing ApplicationsSemantic proximity
Evaluation of Targeted Next-Generation Sequencing for Detection of Bovine Pathogens in Clinical SamplesSemantic proximity
Next-generation sequencing (NGS) in the identification of encephalitis-causing viruses: Unexpected detection of human herpesvirus 1 while searching for RNA pathogensSemantic proximity
The ethics of sequencing infectious disease pathogens for clinical and public healthSemantic proximity
Sequencing from leftover material of stored rapid Ag Tests (Ag-RDTs): a gamechanger in genomic surveillance?Semantic proximity
Evaluating the diagnostic capabilities of nanopore sequencing for <i>Borrelia burgdorferi</i> detection in blacklegged ticksSemantic proximity
Select a neighboring publication to make it the new centre.