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Article

Enhanced expression of PCV2 capsid protein in Escherichia coli and Lactococcus lactis by codon optimization

2011-03-01

Abstract excerpt

Capsid protein (Cap) of porcine circovirus type 2 (PCV2) encoded by orf2 is a main structural protein with strong immunoreactivity. However, capsid protein is expressed poorly in prokaryotic organisms because of differences in codon usage. In this study, we introduce 24 synonymous mutations into orf2 by mutagenic primers and overlap extension polymerase chain reaction (OE-PCR) technique. Fourteen rare codons of or...

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Literature Corpus work
71dc9ac0-2d68-572f-877b-ee39dd936cb7
DOI
10.1007/s11274-010-0503-7
Open publication

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Enhanced expression of PCV2 capsid protein in Escherichia coli and Lactococcus lactis by codon optimizationDOI 10.1007/s11274-010-0503-7
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