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<i>In Ovo</i> Sexing and Genotyping using PCR techniques: A Contribution to the 3R Principles in Chicken Breeding

2025-11-12

Abstract excerpt

Early sex determination and genotyping of chicken embryos is crucial for ethical and resource-efficient animal research, addressing concerns about surplus animals. We developed a reproducible workflow using whole genome amplification combined with Kompetitive Allele Specific PCR (KASP) and standard endpoint PCR to perform in ovo sexing and genotyping from embryonic day four (ED4/96h) onwards. The overall efficien...

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Literature Corpus work
70f613c3-6ac6-53b5-929a-d847f82c11f7
DOI
10.1101/2025.11.11.687802
Open publication

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<i>In Ovo</i> Sexing and Genotyping using PCR techniques: A Contribution to the 3R Principles in Chicken BreedingDOI 10.1101/2025.11.11.687802
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