Article
A genetic quality testing system for early stage embryos in the mouse.
Experimental animals - 1 Oct 2003
Katoh Hideki, Oda Koji, Hioki Kyoji, Muguruma Kaori
Abstract excerpt
We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 x PCR reaction buffer supplemented with proteinase K at a concentration of 40 microg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to...
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