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Article

In ovo sexing and genotyping using PCR techniques: a contribution to the 3R principles in chicken breeding.

Scientific reports - 21 Feb 2026

Dierks C, Förster A, Meunier D, Preisinger R, Klein C, Weigend S, Altgilbers S

Abstract excerpt

Early sex determination and genotyping of chicken embryos is crucial for ethical and resource-efficient animal research. We developed a reproducible workflow using whole genome amplification combined (WGA) with Kompetitive Allele Specific PCR (KASP) and standard endpoint PCR to perform in ovo sexing and genotyping from embryonic day 4 (ED4) to ED10. Across four study phases involving various chicken...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41723204
DOI: 10.1038/s41598-026-40562-y
PMCID: PMC12929762

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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