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Development of an amplicon nanopore sequencing strategy for detection of mutations conferring intermediate resistance to vancomycin in <i>Staphylococcus aureus</i> strains

2022-07-31

Abstract excerpt

Staphylococcus aureus is a major cause of bacteremia and other hospital-acquired infections. The cell-wall active antibiotic vancomycin is commonly used to treat both methicillin-resistant (MRSA) and sensitive (MSSA) infections, but vancomycin intermediate S. aureus (VISA) variants can arise through de novo mutations. Here we performed pilot experiments to develop a combined PCR/long-read sequencing-based method...

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Literature Corpus work
6f31ba58-5bef-5de8-ae5d-f793d4ec3a11
DOI
10.1101/2022.07.31.502206
Open publication

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Development of an amplicon nanopore sequencing strategy for detection of mutations conferring intermediate resistance to vancomycin in <i>Staphylococcus aureus</i> strainsDOI 10.1101/2022.07.31.502206
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