Article
Comparison of Oxford Nanopore Technologies sequencing protocols using R9.4.1 and R10.4.1 flow cells under 20-hour and 48-hour conditions for genotyping methicillin methicillin-resistant Staphylococcus aureus (MRSA).
Molecular biology reports - 14 May 2026
do Couto Mário Sérgio Braga, Helmersen Karin, Deola Aluisio Valerio, Wagner Glauber, Aamot Hege Vangstein, Ferreira Fabienne Antunes
Abstract excerpt
BACKGROUND: Rapid and accurate whole-genome sequencing (WGS) is increasingly important for detecting antimicrobial resistance and virulence determinants in clinical microbiology. Although short-read platforms remain widely used, long-read sequencing technologies have gained relevance due to their ability to resolve complex genomic regions. METHODS AND RESULTS: We analyzed 92 draft genomes generated from 23...
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