Article

Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human-induced pluripotent stem cells

2024-02-02

Abstract excerpt

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an essential tool for gene expression analysis; however, choosing appropriate reference genes for normalization is crucial to ensure data reliability. Most studies on osteogenic differentiation have had limited success in identifying optimal reference genes; to the best of our knowledge, no optimal reference genes in three-dimensio...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6cca06e3-eaa9-57d1-b9f9-fb801e5f21b7
DOI: 10.21203/rs.3.rs-3891485/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human-induced pluripotent stem cellsDOI 10.21203/rs.3.rs-3891485/v1Read

RT-qPCR analyses on the osteogenic differentiation from human iPS cells: An investigation of reference genesSemantic proximity Read

Identification of Stable Reference Genes for Gene Expression Analysis of Three-Dimensional Cultivated Human Bone Marrow-Derived Mesenchymal Stromal Cells for Bone Tissue EngineeringSemantic proximity Read

Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineeringSemantic proximity Read

Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation.Semantic proximity Read

Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilageSemantic proximity Read

TATA box binding protein and ribosomal protein 4 are suitable reference genes for normalization during quantitative polymerase chain reaction study in bovine mesenchymal stem cellsSemantic proximity Read

Postmenopausal Osteoporosis reference genes for qPCR expression assays.Semantic proximity Read

3D encapsulation and inflammatory licensing of mesenchymal stromal cells alter the expression of common reference genes used in real-time RT-qPCRSemantic proximity Read

Housekeeping Gene Stability in Human Mesenchymal Stem and Tendon Cells Exposed to Tenogenic FactorsSemantic proximity Read

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read

Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposureSemantic proximity Read

Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loadingSemantic proximity Read