Article

Accurate and Fast Protein Acylation Identification by Eliminating Position Effects of Cyclic Immonium Ions with Stepped HCD

2025-07-03

Abstract excerpt

Mass spectrometry (MS)-based proteomics is indispensable for studying post-translational modifications (PTMs). Cyclic immonium (CycIm) ions serve as invaluable diagnostic markers for lysine acylations, yet the principles governing their generation efficiency are poorly understood. Here, we systematically investigate this question and uncover a robust “position effect”: the generation of immonium ions is strongly f...

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Literature Corpus work: 6adac8bc-a525-56c5-9592-055edfd3ccef
DOI: 10.1101/2025.07.03.662920

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Accurate and Fast Protein Acylation Identification by Eliminating Position Effects of Cyclic Immonium Ions with Stepped HCDDOI 10.1101/2025.07.03.662920Read

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