Article

Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular tests

2020-06-11

Abstract excerpt

<h4>ABSTRACT</h4> DNA synthesis in vitro has enabled the rapid production of reference standards. These are used as controls, and allow measurement and improvement of the accuracy and quality of diagnostic tests. Current reference standards typically represent target genetic material, and act only as positive controls to assess test sensitivity. However, negative controls are also required to evaluate test speci...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 63d69c05-f512-5651-abca-6bf1a238b388
DOI: 10.1101/2020.06.09.143412

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular testsDOI 10.1101/2020.06.09.143412Read

Patient DNA cross-reactivity of the CDC SARS-CoV-2 extraction control leads to an inherent potential for false negative resultsSemantic proximity Read

Preparation of robust Synthetic Control samples and their use in a metatranscriptomic clinical testSemantic proximity Read

Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal ReferenceSemantic proximity Read

Interlaboratory comparison of SARS-CoV2 molecular detection assays in use by U.S. veterinary diagnostic laboratoriesSemantic proximity Read

Development and Characterization of Synthetic Norovirus RNA for Use in Molecular Detection Methods.Semantic proximity Read

Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example.Semantic proximity Read

External Quality Assessment for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Clinical LaboratoriesSemantic proximity Read

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCRSemantic proximity Read

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR.Semantic proximity Read

Host-virus chimeric events in SARS-CoV2 infected cells are infrequent and artifactualSemantic proximity Read

Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) VirusSemantic proximity Read

Reference materials for SARS-CoV-2 molecular diagnostic quality control: validation of encapsulated synthetic RNAs for room temperature storage and shippingSemantic proximity Read