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Establishment of recombinase polymerase amplification technology for detecting Viral Hemorrhagic Septicemia Virus (VHSV) in cultured aquatic animals

2021-03-01

Abstract excerpt

We established a recombinase polymerase amplification (RPA) assay for the rapid detection of viral hemorrhagic septicemia virus (VHSV) using primers designed to the N gene in this species. Optimization experiments showed that the optimal amplification temperature of the RPA assay was 37 °C and that the reaction could be completed within only 15 min. Specificity tests showed that the RPA assay did not exhibit any c...

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Literature Corpus work
6363a2e3-5fd2-54b5-ac3c-dee4a609d65a
DOI
10.1016/j.aquaculture.2020.736338
Open publication

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Establishment of recombinase polymerase amplification technology for detecting Viral Hemorrhagic Septicemia Virus (VHSV) in cultured aquatic animalsDOI 10.1016/j.aquaculture.2020.736338
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