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Establishment of dual priming oligonucleotide (DPO)-based real-time RT-PCR method for detecting infectious hematopoietic necrosis virus

2025-06-01

Abstract excerpt

Infectious hematopoietic necrosis virus (IHNV) is widely prevalent in the world, causing a large number of deaths in wild and farmed salmonids. Therefore, the establishment of an effective and accurate detection method is extremely important. In this study, the conservation of the N gene of IHNV was analyzed, followed by the design of dual priming oligonucleotide (DPO) for developing the real-time RT-PCR assay. Th...

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Literature Corpus work
31f513a0-9c85-5452-bc38-6d72ca03d287
DOI
10.1016/j.aquaculture.2025.742433
Open publication

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Establishment of dual priming oligonucleotide (DPO)-based real-time RT-PCR method for detecting infectious hematopoietic necrosis virusDOI 10.1016/j.aquaculture.2025.742433
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