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Onsite and visual detection of ranavirus in largemouth bass (Micropterus salmoides) by an isothermal recombinase polymerase amplification method

2022-03-01

Abstract excerpt

Largemouth bass ranavirus (LMBV) has caused mortality in largemouth bass and led to huge economic losses in the aquaculture industry. Here, we developed a novel, fast, and simple isothermal recombinase polymerase amplification assay (RPA) for LMBV detection with primers designed on the basis of a fragment of the major capsid protein gene. The reaction conditions were optimized and the RPA method was specific for L...

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Literature Corpus work
39c1d376-9979-5537-9026-04605fa14a8d
DOI
10.1016/j.aquaculture.2022.737907
Open publication

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Onsite and visual detection of ranavirus in largemouth bass (Micropterus salmoides) by an isothermal recombinase polymerase amplification methodDOI 10.1016/j.aquaculture.2022.737907
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