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Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols.

Diseases of aquatic organisms - 21 Aug 2014

Warg Janet V, Clement Travis, Cornwell Emily R, Cruz Angela, Getchell Rodman G, Giray Cem, Goodwin Andrew E, Groocock Geoffrey H, Faisal Mohamed, Kim Robert, Merry Gwenn E, Phelps Nicholas B D, Reising Monica M, Standish Isaac, Zhang Yan, Toohey-Kurth Kathy

Abstract excerpt

Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method...

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PMID: 25144113
DOI: 10.3354/dao02758

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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