Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development and validation of a reverse transcription quantitative PCR for universal detection of viral hemorrhagic septicemia virus.

Diseases of aquatic organisms - 16 Jun 2011

Garver Kyle A, Hawley Laura M, McClure Carol A, Schroeder Tamara, Aldous Sandra, Doig Fiona, Snow Michael, Edes Sandra, Baynes Catherine, Richard Jon

Abstract excerpt

Viral hemorrhagic septicemia virus (VHSV) infects over 70 fish species inhabiting marine, brackish or freshwater environments throughout the Northern Hemisphere. Over its geographic range, 4 VHSV genotypes and multiple subtypes exist. Here, we describe the development and validation of a rapid, sensitive and specific real-time reverse transcription quantitative PCR assay (RT-qPCR) that amplifies sequence from...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Novirhabdovirus

Share to Reproducibility of Results

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to RNA, Viral

Share to Salmon

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 21848118
DOI: 10.3354/dao02344

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development and validation of a reverse transcription quantitative PCR for universal detection of viral hemorrhagic septicemia virus.PMID 21848118Read ↗

Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols.Semantic proximity Read ↗

Comparison of Quantitative RT-PCR with Cell Culture to Detect Viral Hemorrhagic Septicemia Virus (VHSV) IVb Infections in the Great LakesSemantic proximity Read ↗

Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols.Semantic proximity Read ↗

Validation of a novel one-step reverse transcription polymerase chain reaction method for detecting viral haemorrhagic septicaemia virusSemantic proximity Read ↗

Validation of real time RT-PCR applied to cell culture for diagnosis of any known genotype of viral haemorrhagic septicaemia virus.Semantic proximity Read ↗

Establishment of recombinase polymerase amplification technology for detecting Viral Hemorrhagic Septicemia Virus (VHSV) in cultured aquatic animalsSemantic proximity Read ↗

A novel multiplex RT-qPCR method based on dual-labelled probes suitable for typing all known genotypes of viral haemorrhagic septicaemia virus.Semantic proximity Read ↗

Development of a sensitive and controlled real-time RT-PCR assay for viral haemorrhagic septicaemia virus (VHSV) in marine salmonid aquaculture.Semantic proximity Read ↗

Development and application of quantitative detection method for viral hemorrhagic septicemia virus (VHSV) genogroup IVa.Semantic proximity Read ↗

Partial validation of a TaqMan quantitative polymerase chain reaction for the detection of the three genotypes of Infectious spleen and kidney necrosis virus.Semantic proximity Read ↗

Genotype-specific Taqman® assays for the detection and rapid characterisation of European strains of viral haemorrhagic septicaemia virus.Semantic proximity Read ↗

Real-time RT-PCR for detection, identification and absolute quantification of viral haemorrhagic septicaemia virus using different types of standards.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.