Article

3’ end hydrolysis activity of the Bst polymerase enables rapid and sensitive identification of SARS-CoV-2 variants

2023-03-02

Abstract excerpt

<title>Abstract</title> <p>Sensitive and accurate detection of genetic variants is important in many clinical applications, including the identification of specific pathogens or viral subtypes. However, existing methods are too time-consuming or complex, hampering their deployment in point-of-care settings. Here, we demonstrate that the intrinsic 3’ hydrolysis activity of the Bst polymerase deployed in loop-media...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 603d3715-3fb5-513b-ae95-a02061afd09e
DOI: 10.21203/rs.3.rs-2607942/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

3’ end hydrolysis activity of the Bst polymerase enables rapid and sensitive identification of SARS-CoV-2 variantsDOI 10.21203/rs.3.rs-2607942/v1Read

Non-enzymatic detection of SARS-CoV-2 RNA using DNA nanoswitchesSemantic proximity Read

High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymesSemantic proximity Read

Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assaySemantic proximity Read

Rapid and Multiplexed Nucleic Acid Detection using Programmable Aptamer-Based RNA SwitchesSemantic proximity Read

Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplificationSemantic proximity Read

A Sensitive and Specific Fluorescent RT-LAMP Assay for SARS-CoV-2 Detection in Clinical SamplesSemantic proximity Read

Multiplexed Isothermal Nucleic Acid Detection Using Sequence-Specific Cleavage Mediated by Repair EndonucleasesSemantic proximity Read

Rapid Detection of Intact SARS-CoV-2 Using Designer DNA Nets and a Pocket-Size Smartphone-Linked FluorimeterSemantic proximity Read

Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.Semantic proximity Read

Profiling RT-LAMP tolerance of sequence variation for SARS-CoV-2 RNA detectionSemantic proximity Read

Visual “Dam-Break Drainage” Nucleic Acid Chips: Family-Affordable Platform for Rapid and Ultrasensitive Pathogens DetectionSemantic proximity Read

Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beaconsSemantic proximity Read