Modular DNA Barcoding of Nanobodies Enables Multiplexed <i>in situ</i> Protein Imaging and High-throughput Biomolecule Detection

Abstract excerpt

Current immunodetection methods using antibody-DNA conjugates enable multiplexed target detection through orthogonal DNA barcodes, but existing conjugation approaches are labor-intensive and often compromise antibody function. Here we present a modular, site-specific, and cost-efficient DNA tagging strategy — multiplexed and modular barcoding of antibodies (MaMBA). Utilizing nanobodies as modular adaptors, MaMBA e...

Topics

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• Advanced biosensing and bioanalysis techniques
• Advanced Biosensing Techniques and Applications
• CAR-T cell therapy research
• Cell Image Analysis Techniques
• Chemical Synthesis and Analysis
• Monoclonal and Polyclonal Antibodies Research
• Single-cell and spatial transcriptomics
• T-cell and B-cell Immunology

Identifiers and source

Literature Corpus work

60090e95-5ed4-5d14-95ad-24adbad20c83

DOI

10.1101/2025.01.16.633477