Article

An improved culture protocol for the differentiation and survival of human promyelocytic leukaemia PLB-985 cell-line into mature neutrophil-like granulocytes

2021-09-15

Abstract excerpt

<h4>ABSTRACT</h4> Circulating blood neutrophils are short-lived, lack proliferation capacity and cannot be transfected in vitro to express exogenous genes or proteins. These properties have made the ex vivo genetic manipulation of neutrophils challenging and hindered biochemical and molecular studies investigating the function of specific genes and proteins. Improved methodology for differentiating cell lines i...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5f8475df-4eac-5f2c-ac0b-c5175df1df9b
DOI: 10.1101/2021.09.14.460267

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An improved culture protocol for the differentiation and survival of human promyelocytic leukaemia PLB-985 cell-line into mature neutrophil-like granulocytesDOI 10.1101/2021.09.14.460267Read

A map of gene expression in neutrophil-like cell linesSemantic proximity Read

AN OPTIMIZED METHOD TO DIFFERENTIATE HL60 CELLS INTO NEUTROPHIL-LIKE CELLSSemantic proximity Read

A map of gene expression in neutrophil-like cell linesSemantic proximity Read

Epigenomic and functional dynamics of human bone marrow myeloid differentiation to mature blood neutrophilsSemantic proximity Read

PLB-985 Neutrophil-Like Cells as a Model To Study Aspergillus fumigatus PathogenesisSemantic proximity Read

Studying Neutrophil Function in vitro: Cell Models and Environmental FactorsSemantic proximity Read

Reprogramming of a subpopulation of human blood neutrophils by prolonged exposure to cytokines.Semantic proximity Read

[Phenotype and function of neutrophils in normal human peripheral blood].Semantic proximity Read

IMDM-20 enhances neutrophilic features during DMSO-mediated differentiation of HL-60 cellsSemantic proximity Read

PLB-985 neutrophil-like cells as a model to study <i>Aspergillus fumigatus</i> pathogenesisSemantic proximity Read

Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsSemantic proximity Read

Reparative and regenerative immature neutrophil-like population derived from HL-60 cellsSemantic proximity Read