Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of Bacteroides fragilis

Abstract excerpt

Previously, we reported that metronidazole MICs are not dependent on the expression levels of nim genes in B. fragilis strains; and we also compared the proteomes of metronidazole-resistant laboratory B. fragilis strains to those of their susceptible parent strains. Here, we used RT-qPCR to correlate the expression levels of 18 candidate genes in a panel of selected, clinical nim gene-positive and -negative B. fra...

Topics

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• Bacterial Identification and Susceptibility Testing
• Bacteroides fragilis
• Clostridium difficile and Clostridium perfringens research
• Drug Resistance, Bacterial
• Electrophoresis, Gel, Two-Dimensional
• Fermentation
• Gene Expression Regulation, Bacterial
• Gut microbiota and health

Identifiers and source

Literature Corpus work

5ac15da6-cb4a-5761-920f-0ae0fe6a3c09

DOI

10.20944/preprints202401.1328.v1