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Article

Optimized conditions for GTP loading of Ras.

The Journal of biological chemistry - 1 Dec 2025

Vish Kimberly J, Paul Maxum E, Rollins Asha P, Boggon Titus J

Abstract excerpt

Ras and the small GTPase group are essential for myriad cellular processes and cycle between GDP- and GTP-loaded states to allow stringent control of downstream signaling pathways. Biochemical studies of small GTPases can therefore require a specific nucleotide-bound state. Small GTPases possess basal intrinsic activity to process GTP into GDP plus inorganic phosphate; therefore, in vitro exchange of GDP for GTP...

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Identifiers

PMID: 41207625
DOI: 10.1016/j.jbc.2025.110923
PMCID: PMC12719659

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