Article

Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprinting

2020-01-21

Abstract excerpt

<h4>ABSTRACT</h4> Currently approved therapeutic monoclonal antibodies (mAbs) are based on immunoglobulin G (IgG) 1, 2 or 4 isotypes, which differ in their specific inter-chains disulfide bridge connectivities. Different analytical techniques have been reported for mAb isotyping, among which native ion mobility mass spectrometry (IM-MS) and collision induced unfolding (CIU) experiments. However, mAb isotyping by...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 54f989bc-57f2-531d-a281-604c0e0730ea
DOI: 10.1101/2020.01.20.911750

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprintingDOI 10.1101/2020.01.20.911750Read

Complementary MS methods assist conformational characterization of antibodies with altered S-S bonding networks.Semantic proximity Read

Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural HeterogeneitySemantic proximity Read

Resolving disulfide structural isoforms of IgG2 monoclonal antibodies by ion mobility mass spectrometry.Semantic proximity Read

LC-MS/MS-PRM Quantification of IgG glycoforms using stable isotope labeled IgG1 Fc glycopeptide standardSemantic proximity Read

Immunopeptidomics for Dummies: Detailed Experimental Protocols and Rapid, User-Friendly Visualization of MHC I and II Ligand Datasets with MhcVizPipeSemantic proximity Read

MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc N-GlycosylationSemantic proximity Read

A robust nanoscale RP HPLC-MS approach for sensitive Fc proteoform profiling of IgG allotypesSemantic proximity Read

Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture DissociationSemantic proximity Read

Direct Comparison of the Hinge-Cleaving Proteases IgdE and BdpK for LC-MS-Based IgG1 Clonal ProfilingSemantic proximity Read

Preanalytical Strategies for Native Mass Spectrometry Analysis of Protein Modifications, Complexes, and Higher-Order Structures<b></b>Semantic proximity Read

Unraveling antibody-induced structural dynamics in the ADAMTS13 CUB1-2 domains via HDX-MSSemantic proximity Read

Glycoform Separation and Characterization of Cetuximab Variants by Middle-up Off-Line Capillary Zone Electrophoresis-UV/Electrospray Ionization-MS.Semantic proximity Read