RAPID: an interactive R/Shiny platform for end-to-end 16S rRNA and ITS amplicon sequence analysis using DADA2

Abstract excerpt

<h4>Motivation</h4> Amplicon sequencing of 16S rRNA and internal transcribed spacer (ITS) gene regions is the most widely used approach for characterizing bacterial and fungal communities, respectively. The DADA2 pipeline has become a standard for inferring amplicon sequence variants (ASVs), offering single-nucleotide resolution over traditional OTU clustering. However, executing the full DADA2 workflow requires...

Topics

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• Algorithms
• Computational Biology
• Databases, Genetic
• DNA, Ribosomal Spacer
• Genetic Variation
• High-Throughput Nucleotide Sequencing
• Microbiota
• Software

Identifiers and source

Literature Corpus work

52c3fac8-2b7c-536f-aacd-7202204f3cce

DOI

10.64898/2026.05.05.723040