Article

One is not enough: on the effects of reference genome for the mapping and subsequent analyses of short-reads

2020-04-14

Abstract excerpt

Mapping of high-throughput sequencing (HTS) reads to a single arbitrary reference genome is a frequently used approach in microbial genomics. However, the choice of a reference may represent a source of errors that may affect subsequent analyses such as the detection of single nucleotide polymorphisms (SNPs) and phylogenetic inference. In this work, we evaluated the effect of reference choice on short-read sequenc...

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Literature Corpus work: 5076ec7a-81cc-5c34-a945-c107ccc72ad0
DOI: 10.1101/2020.04.14.041004

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