Article

A secreted proteomic footprint for stem cell pluripotency

2021-04-14

Abstract excerpt

With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the spent medium from cultured embryonic (Man-13) and induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintain pluripotency, and then transferred to FGF2 and TGFβ deficient media for 48 hours to replicat...

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Literature Corpus work: 4f7c6d8e-9935-59a5-b1db-8fc3f0d1235b
DOI: 10.1101/2021.04.14.439804

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