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Selective whole genome amplification as a tool to enrich specimens with low <i>Treponema pallidum</i> genomic DNA copies for whole genome sequencing

2021-07-10

Abstract excerpt

Downstream next generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum ( T. pallidum ) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. In this study, we investigated selective whole genome amplification (SWGA) utilizing multiple displacement amplification (MDA) in conjunction with custom oligonucleotides...

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Literature Corpus work
4c93083c-a306-5ac5-8482-8fd330fc65c0
DOI
10.1101/2021.07.09.451864
Open publication

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