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Optimizing Sample Preparation for Direct Nanopore Sequencing to Enable Rapid Pathogen and Antimicrobial Resistance Profiling in Bovine Mastitis

2025-08-19

Abstract excerpt

Long-read metagenomic sequencing allows for the rapid, culture-independent, and accurate identification of causative pathogens and antimicrobial resistance (AMR) profiles, supporting precise antibiotic use and reducing the spread of resistance. However, its application to mastitis milk is challenging due to the complex milk matrix, low bacterial count, and high somatic cell content. This study primarily aimed to f...

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Literature Corpus work
4c325c39-1ce9-5f3e-b76d-07b539deb7c8
DOI
10.1101/2025.08.19.671071
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Optimizing Sample Preparation for Direct Nanopore Sequencing to Enable Rapid Pathogen and Antimicrobial Resistance Profiling in Bovine MastitisDOI 10.1101/2025.08.19.671071
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