Article

Spatial total RNA sequencing of formalin-fixed paraffin embedded tissue by spRandom-seq

2025-09-07

Abstract excerpt

<title>Abstract</title> <p>Current sequencing-based spatial transcriptomic methods have been widely used to resolve gene expression; however, these methods are limited to fresh or fresh-frozen samples due to the low efficiency of oligo(dT) primers in degraded RNA samples. Here, we have developed a random primer-based spatial total RNA sequencing (spRandom-seq) technology for Formalin-fixed paraffin-embedded (FFPE...

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Literature Corpus work: 448d2f69-6137-59f0-b914-1b8d9ecb0615
DOI: 10.21203/rs.3.rs-3987790/v1

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