Article

Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655

2020-05-29

Abstract excerpt

<title>Abstract</title> <p>Based on the analysis of CpxP genes among Escherichia coli strains, CpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1655 (pCas9). The CpxP gene expression cassette was amplified by PCR and subcloned into pB...

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Literature Corpus work: 42cfa5f6-0fca-575e-a0d5-a66cde47b180
DOI: 10.21203/rs.3.rs-31252/v1

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