Article

Impact of the size of CRISPR-Cas gene cassettes on the packaging efficiency and transduction titer of lentiviral vectors

2022-03-29

Abstract excerpt

<h4>Background: </h4> CRISPR-Cas technology has revolutionized the use of genome-editing in studies on diverse biological topics. Lentiviral vectors (LVs) have been widely used for the generation of stably transduced cells that durably express the Cas protein and guide RNA (gRNA), the key components of the CRISPR-Cas editing system. This delivery system allows sustained gene expression in dividing and non-dividing...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3e568f74-540c-54dc-b1ef-f383cb0fafde
DOI: 10.21203/rs.3.rs-1458483/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Impact of the size of CRISPR-Cas gene cassettes on the packaging efficiency and transduction titer of lentiviral vectorsDOI 10.21203/rs.3.rs-1458483/v1Read

Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and PerspectivesSemantic proximity Read

Lentiviral vector packaging and producer cell lines yield titers equivalent to the industry-standard four-plasmid processSemantic proximity Read

Transient Retrovirus-Based CRISPR/Cas9 All-in-One Particles for Efficient, Targeted Gene KnockoutSemantic proximity Read

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection.Semantic proximity Read

Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTRSemantic proximity Read

Development of Lentiviral Packaging Cells and Scale Up of Production to Meet the Growing Demand in Cell and Gene TherapySemantic proximity Read

Overcoming lentiviral delivery limitations in hard-to-transduce suspension cells for genome-wide CRISPR screeningSemantic proximity Read

Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 yearsSemantic proximity Read

Development of Stable Packaging and Producer Cell Lines for the Production of AAV VectorsSemantic proximity Read

Delivery approaches for CRISPR/Cas9 therapeutics in vivo: advances and challengesSemantic proximity Read

Detailed Protocol for the Novel and Scalable Viral Vector Upstream Process for AAV Gene Therapy ManufacturingSemantic proximity Read

CRISPR screening of porcine sgRNA library identified host factors essential for Japanese encephalitis virus replicationSemantic proximity Read