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Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTR

21 Apr 2009

Abstract excerpt

Self-inactivating (SIN) lentiviruses flanked by the 1.2-kb chicken hypersensitive site-4 (cHS4) insulator element provide consistent, improved expression of transgenes, but have significantly lower titers. The mechanism by which this occurs is unknown. Lengthening the lentiviral (LV) vector transgene cassette by an additional 1.2 kb by an internal cassette caused no further reduction in titers. However, when cHS4...

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PMID: 19384292
DOI: 10.1038/mt.2009.89

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