Article

Gene-expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ

2017-10-27

Abstract excerpt

RNA sequencing (RNA-seq) is a sensitive and accurate method for quantifying gene expression. Small samples or those whose RNA is degraded, such as formalin-fixed, paraffin-embedded (FFPE) tissue, remain challenging to study with nonspecialized RNA-seq protocols. Here we present a new method, Smart-3SEQ, that accurately quantifies transcript abundance even with small amounts of total RNA and effectively characteriz...

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Literature Corpus work: 39d5cac9-cc67-58aa-80e2-49c35999577c
DOI: 10.1101/207340

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